ABSTRACT
Objective@#To investigate the clincopathologic and immunohistochemical features of gastric glomus tumors and their differences from gastric neuroendocrine neoplasms.@*Methods@#Six cases of gastric glomus tumors, 8 cases of glomus tumors in other sites and 7 cases of gastric neuroendocrine neoplasms were collected from the Department of Pathology, Taizhou Hospital. The clinicopathological and immunohistochemical characteristics of these tumors were analyzed retrospectively.@*Results@#The gastric glomus tumors were located in the muscularis propria of the antrum and most cases strongly expressed synaptophysin (5/6). However, no synaptophysin expression was seen in glomus tumors of other organs.Most gastric neuroendocrine neoplasms were located in the mucosa or submucosa of the fundus and corpus. In addition to the strong expression of synaptophysin (7/7), CgA (6/7) and CD56(5/7) were strongly positive, although SMA was negative.@*Conclusions@#Gastric glomus tumors and neuroendocrine neoplasms have similar morphological characteristics and both show strongly expression of synaptophysin. However, the location and immunohistochemical characteristics of gastric glomus tumors differ from those of the neuroendocrine neoplasms.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate neuroendocrine differentiation and Wilms' tumor protein-1 (WT-1) expression in breast mucinous carcinoma and their clinicopathological significance.</p><p><b>METHODS</b>The clinicopathological data of 65 patients with breast mucinous carcinoma, including 31 cases of mixed mucinous carcinoma, 23 cases of hypocellular pure mucinous carcinoma and 11 cases of hypercellular pure mucinous carcinoma, admitted in Taizhou Hospital from January 2010 to June 2015 were retrospectively reviewed. The expression of neuroendocrine markers and WT-1 was detected by immunohistochemistry staining in all cases.</p><p><b>RESULTS</b>The mixed mucinous carcinomas and hypercelluar pure mucinous carcinomas had higher incidence of axillary lymph node metastasis and human epidermal recepter 2 (HER-2) positive than hypocellular pure mucinous carcinoma (all (P<0.01). However, the difference was not significant between mixed mucinous carcinomas and hypercellular pure mucinous carcinomas (all P>0.05). The expression of neuroendocrine marker was stronger in hypercellular mucinous carcinoma than that in mixed mucinous carcinoma and hypocellular mucinous carcinoma (all (P<0.05), but the difference was not statistically significant between mixed mucinous carcinoma and hypocellular pure mucinous carcinoma (P>0.05). The expression of WT-1 was weaker in mixed mucinous carcinoma than that in hypercellular and hypocellular pure mucinous carcinoma(all (P<0.05), but the difference was not statistically significant between hypercellular and hypocellular pure mucinous carcinoma (P>0.05). The mucinous carcinomas with lymph node metastasis had lower expression of neuroendocrine markers than those without lymph node metastasis ((P<0.01). The expression of WT-1 in breast mucinous carcinoma with lymph node metastasis trended lower than that in those without lymph node metastasis, but the difference was not statistically significant (P>0.05).</p><p><b>CONCLUSION</b>Hypercellular pure mucinous breast carcinoma has higher rates of lymph node metastasis and HER-2 amplification than hypocellular pure mucinous carcinoma, the sub-classification of breast pure mucinous carcinoma should be considered. Neuroendocrine differentiation and WT-1 expression may be helpful in distinguishing the subtypes of breast mucinous carcinoma. Breast mucinous carcinoma with neuroendocrine differentiation trends to have less lymph node metastasis.</p>
Subject(s)
Female , Humans , Adenocarcinoma, Mucinous , Classification , Diagnosis , Pathology , Axilla , Breast Neoplasms , Classification , Diagnosis , Pathology , Immunohistochemistry , Incidence , Lymph Nodes , Pathology , Lymphatic Metastasis , Neuroendocrine Tumors , Diagnosis , Pathology , Receptor, ErbB-2 , Metabolism , Retrospective Studies , WT1 Proteins , MetabolismABSTRACT
The clinicopathological data of 13 patients with Langerhans cell histiocytosis (LCH) from January 2004 to January 2014 were retrospectively analyzed.There were 8 males and 5 females with a mean age of 35 (6-63)years.For all 13 cases,there were 15 lesions.Histologically,there was a diffuse distribution of Langerhans cells accompanied by a variable number of eosinophils and some other inflammatory cells.Immunohistochemical study showed tumor cells were positive for S-100 protein,CD1a and Langerin.During a follow-up period of 6-101 months,all survived.The diagnosis of LCH was assisted by immunophenotypic analyses for S-100,CD1a and Langerin.Combination therapy was effective.Most patients had an excellent prognosis.
ABSTRACT
Objective To study the expression of collagenase Ⅳ and collagen type Ⅳ in papillary thyroid carcinoma (PTC). Methods Immunohistochemical staining(SP method)was used to detect the expression of collagenase Ⅳ and collagen typeⅣ. Results ⑴The positive expression rate of collagenase Ⅳ was 77 9%, 66 7%, 80 0%, 88 9% and 25 4% in PTC, papillary microcarcinoma, intra-thyroid, extra-thyroid carcinoma and tissues adjacent to tumor(P
ABSTRACT
Objective To investigate the significance of metallothionein (MT) expression in the placenta of women exposed to low level lead during pregnancy. Methods Sixty-seven pregnant women with blood lead level ranging from 1.5 ?mol/L to 4.8 ?mol/L were randomly selected from the Department of Obstetrics of Qingdao Municipal Hospital between Mar 2005 and Mar 2006. Among them, 35 were with blood lead level less than 2.9 ?mol/L(group A) and 32 more than 2.9 ?mol/L(group B). Immunohistochemical streptavidin-peroxidase-biotin methods were used to observe the expression of MT in the placental tissue. Results (1)Among the 67 pregnant women,the highest level of blood lead was 4.7 ?mol/L , and the lowest level was 1.6 ?mol/L .The blood lead level of groups A and B was (1.7?0.3) ?mol/L, and (3.1?0.4) ?mol/L, with a significant difference between them(P